pfge chromosome size standards Search Results


dna marker  (New England Biolabs)
96
New England Biolabs dna marker
Dna Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pmc09064518-89-11-15?v=New+England+Biolabs
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s lambda pfg dna markers  (New England Biolabs)
96
New England Biolabs s lambda pfg dna markers
S Lambda Pfg Dna Markers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/10__1128_slash_jcm__39__3__1057___1066__2001-83-21-26?v=New+England+Biolabs
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chromosomal grade agarose  (Bio-Rad)
96
Bio-Rad chromosomal grade agarose
Chromosomal Grade Agarose, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pmc00129033-444-7-10?v=Bio-Rad
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chromosomal grade agarose - by Bioz Stars, 2026-08
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high molecular weight dna marker  (Thermo Fisher)
99
Thermo Fisher high molecular weight dna marker
Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. <t>DNA</t> fragments EF and FE were ligated and analyzed <t>by</t> <t>PFGE</t> in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.
High Molecular Weight Dna Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pmc00114390-71-15-18?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
high molecular weight dna marker - by Bioz Stars, 2026-08
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chromosomal dna  (New England Biolabs)
96
New England Biolabs chromosomal dna
Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. <t>DNA</t> fragments EF and FE were ligated and analyzed <t>by</t> <t>PFGE</t> in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.
Chromosomal Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pm15356810-60-16-23?v=New+England+Biolabs
Average 96 stars, based on 1 article reviews
chromosomal dna - by Bioz Stars, 2026-08
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standard dna marker  (New England Biolabs)
95
New England Biolabs standard dna marker
Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. <t>DNA</t> fragments EF and FE were ligated and analyzed <t>by</t> <t>PFGE</t> in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.
Standard Dna Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pm25764567-61-1-8?v=New+England+Biolabs
Average 95 stars, based on 1 article reviews
standard dna marker - by Bioz Stars, 2026-08
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xbai  (New England Biolabs)
99
New England Biolabs xbai
Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. <t>DNA</t> fragments EF and FE were ligated and analyzed <t>by</t> <t>PFGE</t> in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.
Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pm11716634-73-17-18?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
xbai - by Bioz Stars, 2026-08
99/100 stars
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dna molecular size standards for pfge  (New England Biolabs)
96
New England Biolabs dna molecular size standards for pfge
Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. <t>DNA</t> fragments EF and FE were ligated and analyzed <t>by</t> <t>PFGE</t> in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.
Dna Molecular Size Standards For Pfge, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pm17110978-66-16-43?v=New+England+Biolabs
Average 96 stars, based on 1 article reviews
dna molecular size standards for pfge - by Bioz Stars, 2026-08
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pulsed field gel electrophoresis pfge analysis f chromosomal restriction fragments  (New England Biolabs)
96
New England Biolabs pulsed field gel electrophoresis pfge analysis f chromosomal restriction fragments
Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. <t>DNA</t> fragments EF and FE were ligated and analyzed <t>by</t> <t>PFGE</t> in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.
Pulsed Field Gel Electrophoresis Pfge Analysis F Chromosomal Restriction Fragments, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+chromosome+size+standards/pm24560919-46-10-23?v=New+England+Biolabs
Average 96 stars, based on 1 article reviews
pulsed field gel electrophoresis pfge analysis f chromosomal restriction fragments - by Bioz Stars, 2026-08
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Image Search Results


Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. DNA fragments EF and FE were ligated and analyzed by PFGE in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.

Journal:

Article Title: Viral Replicase Gene Products Suffice for Coronavirus Discontinuous Transcription

doi: 10.1128/JVI.75.14.6676-6681.2001

Figure Lengend Snippet: Cloning of HCoV-vec cDNA in the vaccinia virus genome. (a) Assembly of a 22.5-kbp HCoV-vec cDNA by in vitro ligation. DNA fragments EF and FE were ligated and analyzed by PFGE in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany). The ligation substrates and products are illustrated. The relevant restriction sites and the sizes of DNA fragments are shown. AP indicates treatment of DNA fragment ends with alkaline phosphatase. (b) Forced ligation of recombinant vaccinia virus genomes. PFGE analysis of the ligation reaction containing EagI-cleaved, dephosphorylated HCoV-vec insert cDNA and NotI-cleaved vaccinia virus vNotI/tk DNA in the presence of NotI enzyme is shown. The accumulated ligation products, comprised of two vaccinia virus DNA arms and a copy of the HCoV-vec insert cDNA (long/insert/long, long/insert/short, or short/insert/short), as well as relevant substrates and intermediate ligation products, are indicated. DNA bands that are not indicated represent substrates and predicted minor ligation products comprised of pEB, PCR-BF, pFE, and vNotI/tk DNA fragments. (c) Southern blot analysis of 10 random vaccinia virus vHCoV-vec clones. DNA from CV-1 cells infected with vNotI/tk (lane vNot) or recombinant vHCoV-vec clones was digested with HindIII and analyzed by Southern blotting with a random-primed 32P DNA probe representing the HCoV 229E genome between nt 1048 and 20582. Lane M shows a HindIII-digested DNA fragment generated by RT-PCR from HCoV 229E poly(A)-containing RNA. This 19.5-kbp cDNA fragment represents the HCoV genome between nt 1048 and 20582. The two patterns of hybridization (cf. lanes 1 to 6 and 9 with lanes 7, 8, and 10) represent the two possible insert orientations.

Article Snippet: DNA fragments EF and FE were ligated and analyzed by PFGE in comparison with a high-molecular-weight DNA marker (Life Technologies, Karlsruhe, Germany).

Techniques: Clone Assay, In Vitro, Ligation, Molecular Weight, Marker, Recombinant, Southern Blot, Infection, Random Primed, Generated, Reverse Transcription Polymerase Chain Reaction, Hybridization